site > International journal > (1997) Fed-batch fermentation of recombinant Escherichia coli to produce Bacillus macerans CGTase. Journal of Microbiology and Biotechnology 7(5): 323-328 ,1997

Dept. of Advanced Fermentation Fusion Science & Technology, Kookmin University

|
회원가입

Topics

Professor | Current members | Alumni

Topics

International | Domestic | Patent

HOME > PUBLICATIONS

PUBLICATIONS

작성일 : 12-07-17 13:01

(1997) Fed-batch fermentation of recombinant Escherichia coli to produce Bacillus macerans CGTase. Journal of Microbiology and Biotechnology 7(5): 323-328 ,1997

글쓴이 : 박용철

추천 : 0

http://onlinelibrary.wiley.com/doi/10.1042/BA19990065/abstract;jsessio… [401]

Fed-batch fermentation of recombinant Escherichia coli to produce Bacillus macerans CGTase.

Yong-Cheol Park, Chang-Sup Kim, Chung-Im Kim, Kyu-Hwan Choi and Jin-Ho Seo*
Journal of Microbiology and Biotechnology 7(5): 323-328 ,1997
(SCI I/F:1.381)

Abstract
The recombinant Escherichia coli BL21(DE3)pLysE:pTCGT1 was grown to overprodude Bacillus macerans cyclodextrin glucanotransferase (CGTase) able to synthesize α-cyclodextrin (CD) with a selectivity of 67%. A number of batch fermentations were performed to test the possibility of using lactose as an inducer of the E.coli T7 promoter system. A mixture of isopropy1 β-D-thiogalactoside (IPTG) and lactose (1:1) gave a maximum CGTase activity of 2.4 U/ml, which was higher than the value obtained with induction by IPTG alone. Fed-batch fermentations involving a glucose-controlled growth period followed by a gene-expression phase with mixtures of IPTG and lactose were employed to achieve high cell density and thereby increase total CGTase activity. Optimized fed-batch fermentation using the modified inducer (IPTG:lactose = 1:3) and 100 g/l yeast extract solution in the gene-expression phase resulted in a maximum CGTase activity of 62.9 U/ml and a final cell mass 53.5 g/l, corresponding to a 31-fold increase in CGTase activity and a 29-fold increase in cell mass compared with the control batch fermentation.

Contact Us

Science Building Room No. 204-205, Kookmin University
861-1 Jeongrung-dong, Sungbuk-gu, Seoul 136-702
Tel: +82-2-910-5596(5462), Fax: +82-2-910-5739
E-mail: ycpark@kookmin.ac.kr