site > International journal > (2004) Fed-batch production of D-ribose from sugar mixtures by transketolase deficient Bacillus subtilis SPK1.

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작성일 : 12-07-17 16:54

(2004) Fed-batch production of D-ribose from sugar mixtures by transketolase deficient Bacillus subtilis SPK1.

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http://www.springerlink.com/content/g4l0lnrgmkjmwbun/ [432]

Fed-batch production of D-ribose from sugar mixtures by transketolase deficient Bacillus subtilis SPK1.

Yong-Cheol Park, Sung-Gun Kim, Kyung-Moon Park, Kelvin H. Lee and Jin-Ho Seo*
Applied Microbiology and Biotechnology 66(3): 297-302. 2004.12.30.
(SCI I/F:3.425)

d-Ribose, a five-carbon sugar, is used as a key intermediate for the production of various biomaterials, such as riboflavin and inosine monophosphate. A high d-ribose-producing Bacillus subtilis SPK1 strain was constructed by the chemical mutation of the transketolase-deficient strain, B. subtilis JY1. Batch fermentation of B. subtilis SPK1 with 20 g l–1 xylose and 20 g l–1 glucose resulted in 4.78 g l–1 dry cell mass, 23.0 g l–1d-ribose concentration, and 0.72 g l–1 h–1 productivity, corresponding to a 1.5- to 1.7-fold increase when compared with values for the parental strain. A late-exponential phase was chosen as the best point for switching to a fed-batch process. Optimized fed-batch fermentation of B. subtilis SPK1, feeding a mixture of 200 g l–1 xylose and 50 g l–1 glucose after the late-exponential phase reduced the residual xylose and glucose concentrations to less than 7.0 g l–1 and gave the best results of 46.6 g l–1d-ribose concentration and 0.88 g l–1 h–1 productivity which were 2.0- and 1.2-fold higher than the corresponding values in a simple batch fermentation.

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