site > International journal > (2004) Production of xylitol by recombinant Saccharomyces cerevisiae containing xylose reductase gene in repeated fed-batch and cell-recycle fermentation.

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작성일 : 12-07-17 16:52

(2004) Production of xylitol by recombinant Saccharomyces cerevisiae containing xylose reductase gene in repeated fed-batch and cell-recycle fermentation.

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http://www.sciencedirect.com/science/article/pii/S0141022904002236 [1112]

Production of xylitol by recombinant Saccharomyces cerevisiae containing xylose reductase gene in repeated fed-batch and cell-recycle fermentation.

Sun-Myung Bae, Yong-Cheol Park, Tae-Hee Lee, Do-Hyun Kweon, Jin-Ho Choi, Sung-Koo Kim, Yeon-Woo Ryu and Jin-Ho Seo*
Enzyme and Microbial Technology 35(6-7): 545-549. 2004.12.01.
(SCI I/F: 2.367)

Xylitol is a well-known sugar substitute with low-calorie and anti-cariogenic characteristics. An effort of biological production of xylitol from xylose was made in repeatedfed-batch and cell-recycle fermentations of recombinantSaccharomycescerevisiae BJ3505/δXR harboring the xylosereductasegene from Pichia stipitis. Batchfermentation with 20 g/l xylose and 18 g/l glucose resulted in 9.52 g/l dry cell mass, 20.1 g/l xylitol concentration and approximately 100% conversion yield. Repeatedfed-batch operation to remove 10% of culture broth and to supplement an equal volume of 200 g/l xylose was designed to improve xylitolproduction. In spite of a sudden drop of cell concentration, an increase in dry cell mass led to high accumulation of xylitol at 48.7 g/l. To overcome loss of xylitol-producing biocatalysts in repeatedfed-batchfermentation, cell-recycle equipment of hollow fiber membrane was implemented into a xylitolproduction system. Cell-recycle operation maintained concentration of the recombinantcells high inside a bioreactor. Final dry cell mass of 22.0 g/l, 116 g/l xylitol concentration, 2.34 g/l h overall xylitol productivity were obtained in cell-recyclefermentation supplemented with xylose and yeast extract solution, which were equivalent to 2.3-, 5.8- and 3.8-fold increases compared with the corresponding values of batch-type xylitolproduction parameters.

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