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(2004) Optimization of culture conditions for D-ribose production by transketolase deficient Bacillus subtilis JY1

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http://cat.inist.fr/?aModele=afficheN&cpsidt=16064661 [404]

Optimization of culture conditions for D-ribose production by transketolase deficient Bacillus subtilis JY1

Yong-Cheol Park and Jin-Ho Seo*
Journal of Microbiology and Biotechnology 14(4): 665-672. 2004.08.28.
(SCI I/F: 1.381)

D-Ribose is a five-carbon sugar used for the commercial synthesis of riboflavin, antiviral agents, and flavor enhancers. Batch fermentations with transketolase-deficient B. subtilis JY 1 were carried out to optimize the production of D-ribose from xylose. The best results for the fermentation were obtained with a temperature of 37C and an initial pH of 7.0. Among various sugars and sugar alcohols tested, glucose and sucrose were found to be the most effective for both cell growth and D-ribose production. The addition of 15 g/l xylose and 15 g/l glucose improved the fermentation performance, presumably due to the adequate supply of ATP in the xylose metabolism from D-xylulose to D-xylulose-5-phosphate. A batch culture in a 3.7-1 jar fermentor with 14.9 g/l xylose and 13.1 g/l glucose resulted in 10.1 g/l D-ribose concentration with a yield of 0.62 g D-ribose/g sugar consumed, and 0.25 g/ 1-h of productivity. Furthermore, the sugar utilization profile, indicating the simultaneous consumption of xylose and glucose, and respiratory parameters for the glucose and sucrose media suggested that the transketolase-deficient B. subtilis JY1 lost the glucose-specific enzyme II of the phosphoenolpyruvate transferase system.

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