site > International journal > (2000) Refolding of Bacillus macerans cyclodextrin glucanotransferase expressed as inclusion bodies in recombinant Escherichia coli.

Dept. of Advanced Fermentation Fusion Science & Technology, Kookmin University

|
회원가입

Topics

Professor | Current members | Alumni

Topics

International | Domestic | Patent

HOME > PUBLICATIONS

PUBLICATIONS

작성일 : 12-07-17 14:06

(2000) Refolding of Bacillus macerans cyclodextrin glucanotransferase expressed as inclusion bodies in recombinant Escherichia coli.

글쓴이 : 박용철

추천 : 0

http://cat.inist.fr/?aModele=afficheN&cpsidt=801161 [6896]

Refolding of Bacillus macerans cyclodextrin glucanotransferase expressed as inclusion bodies in recombinant Escherichia coli.

Chung-Im Kim, Myoung-Dong Kim, Yong-Cheol Park, Nam Soo Han and Jin-Ho Seo*
Journal of Microbiology and Biotechnology 10(5): 632-637. 2000.10.31.
(SCI I/F: 1.381)

This research was undertaken to restore the biological activity of cyclodextrin glucanotransferase (CGTase) of Bacillus macerans origin expressed as inclusion bodies in recombinant Escherichia coli. The optimum concentration of urea used as a denaturant was 8 M. The supplementation of 0.5 M urea into a dialysis buffer increased the refolding efficiency by preventing any protein aggregation. The influence of the protein concentration, temperature, and pH were also investigated. The protein concentration was found to be the most important factor in the refolding efficiency. The optimum temperature was 15-25°C and the optimum pH was 6.0. The maximum specific activity of the CGTase refolded under the optimum conditions was 92.2 U/mg, corresponding to 72% of the native CGTase. A comparison of the secondary structure between the native and the refolded CGTases showed that the relative ratio of the α-helix content in the native to the refolded CGTase was 1:0.82.

Contact Us

Science Building Room No. 204-205, Kookmin University
861-1 Jeongrung-dong, Sungbuk-gu, Seoul 136-702
Tel: +82-2-910-5596(5462), Fax: +82-2-910-5739
E-mail: ycpark@kookmin.ac.kr