site > International journal > (2009) Expression of hepatitis B surface antigen S domain in recombinant Saccharomyces cerevisiae using GAL1 promoter.

Dept. of Advanced Fermentation Fusion Science & Technology, Kookmin University

|
회원가입

Topics

Professor | Current members | Alumni

Topics

International | Domestic | Patent

HOME > PUBLICATIONS

PUBLICATIONS

작성일 : 12-07-17 18:00

(2009) Expression of hepatitis B surface antigen S domain in recombinant Saccharomyces cerevisiae using GAL1 promoter.

글쓴이 : 박용철

추천 : 0

http://www.sciencedirect.com/science/article/pii/S0168165609001291 [1216]

Expression of hepatitis B surface antigen S domain in recombinant Saccharomyces cerevisiae using GAL1 promoter.

Eun-Jeong Kim, Young-Kyoung Park, Hyung-Kweon Lim, Yong-Cheol Park* and Jin-Ho Seo*
Journal of Biotechnology 141(3-4): 155-159 2009.05.20.
(SCI I/F: 3.045)

HepatitisB virus, a member of the hepadnavirus family, causes the acute and chronic diseases of the human liver. The Sdomain of hepatitisB virus surfaceantigen (sHBsAg) was expressed under the control of the galactose-inducible GAL1promoter in recombinantSaccharomycescerevisiae. Batch fermentation of S. cerevisiae 2805/pδMFα-sHBsAg resulted in 4.92 g l−1 dry cell mass and 2.21 mg l−1 sHBsAg concentration. To improve the expression level of sHBsAg, the pdi1 gene encoding protein disulfide isomerase was coexpressed in recombinantS. cerevisiae. Batch fermentation of S. cerevisiae 2805/pδMFα-sHBsAg + pPDI using 21 g l−1 glucose and 33 g l−1 galactose resulted in 9.75 g l−1 dry cell mass and 13.3 mg l−1 sHBsAg concentration, which were 2 and 6 times higher than those for S. cerevisiae 2805/pδMFα-sHBsAg, respectively. Appearance of three sHBsAgs with different molecular sizes of 24 kDa, 34 kDa and 40 kDa in immunoblot assay and their endoglycosidase treatment indicated that sHBsAg might be expressed in three types of the authentic, MFα signal sequence-containing and N-glycosylated MFα signal sequence-containing forms. Fed-batch fermentation of recombinantS. cerevisiae 2805 coexpressing the sHBsAg and pdi1 genes was carried out by feeding 600 g l−1 glucose continuously and controlling galactose concentration at around 20 g l−1. As a result, 20.2 g l−1 dry cell mass and 74.4 mg l−1 maximum sHBsAg concentration were obtained, which were 4.1 and 33.7 times higher than those for the batch fermentation of S. cerevisiae 2805/pδMFα-sHBsAg, respectively.

Contact Us

Science Building Room No. 204-205, Kookmin University
861-1 Jeongrung-dong, Sungbuk-gu, Seoul 136-702
Tel: +82-2-910-5596(5462), Fax: +82-2-910-5739
E-mail: ycpark@kookmin.ac.kr