site > International journal > (2008) Proteomics analysis of fructophilic properties of osmotolerant Candida agnolia.

Dept. of Advanced Fermentation Fusion Science & Technology, Kookmin University

|
회원가입

Topics

Professor | Current members | Alumni

Topics

International | Domestic | Patent

HOME > PUBLICATIONS

PUBLICATIONS

작성일 : 12-07-17 17:46

(2008) Proteomics analysis of fructophilic properties of osmotolerant Candida agnolia.

글쓴이 : 박용철

추천 : 0

http://www.ncbi.nlm.nih.gov/pubmed/18309268 [971]

http://www.jmb.or.kr/journal/viewJournal.html?year=2008&vol=18&num=2&p… [432]

Proteomics analysis of fructophilic properties of osmotolerant Candida agnolia.

Ji-Hee Yu, Dae-Hee Lee, Yong-Cheol Park, Mi-Gi Lee, Dae-Ok Kim, Yeon-Woo Ryu and Jin-Ho Seo*
Journal of Microbiology and Biotechnology 18(2): 248-254. 2008.02.28.
(SCI I/F: 1.381)

Candida magnoliae, an osmotolerant and erythritol producing yeast, prefers D-fructose to D-glucose as carbon sources. For the investigation of the fructophilic characteristics with respect to sugar transportation, a sequential extraction method using various detergents and ultracentrifugation was developed to isolate cellular membrane proteins in C. magnoliae. Immunoblot analysis with the Pma1 antibody and twodimensional electrophoresis analysis coupled with MS showed that the fraction II was enriched with membrane proteins. Eighteen proteins out of 36 spots were identified as membrane or membrane-associated proteins involved in sugar uptake, stress response, carbon metabolism, and so on. Among them, three proteins were significantly upregulated under the fructose supplying conditions. The hexose transporter was highly homologous to Ght6p in Schizosaccharomyces pombe, which was known as a predominant transporter for the fructose uptake of S. pombe because it exhibited higher affinity to D-fructose than D-glucose. The physicochemical properties of the ATP-binding cassette transporter and inorganic transporter explained their direct or indirect associations with the fructophilic behavior of C. magnoliae. The identification and characterization of membrane proteins involved in sugar uptake might contribute to the elucidation of the selective utilization of fructose to glucose by C. magnoliae at a molecular level.

Contact Us

Science Building Room No. 204-205, Kookmin University
861-1 Jeongrung-dong, Sungbuk-gu, Seoul 136-702
Tel: +82-2-910-5596(5462), Fax: +82-2-910-5739
E-mail: ycpark@kookmin.ac.kr