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작성일 : 12-07-17 17:34

(2006) Characterization of D-ribose biosynthesis in Bacillus subtilis JY200 deficient in transketolase gene.

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http://www.sciencedirect.com/science/article/pii/S0168165605004773 [1006]

Characterization of D-ribose biosynthesis in Bacillus subtilis JY200 deficient in transketolase gene.

Yong-Cheol Park, Jin-Ho Choi, George N. Bennett and Jin-Ho Seo*
Journal of Biotechnology 121(4): 508-516. 2006. 02. 24.
(SCI I/F: 3.045)

d-Ribose is a functional five-carbon sugar, which has been used for the commercial production of riboflavin. Mechanisms of d-ribosebiosynthesis from xylose were investigated in the genetically engineered BacillussubtilisJY200 with a deficiency in transketolase. A transketolasegene (tkt) disruption cassette in plasmid pUNKC was introduced into the chromosomal tktgene in the wild type B. subtilis 168. Analysis of culture broth by thin layer chromatography confirmed that the disruption of tkt allowed B. subtilisJY200 to produce d-ribose. In a batch culture of B. subtilisJY200, a loss of cell viability was observed after glucose depletion. Fed-batch cultivation by feeding 400 g l−1 glucose solution as a co-substrate was carried out to supply energy to xylose metabolism and to maintain cell viability throughout cultivation. Fed-batch cultivation of B. subtilisJY200 in a complex medium containing 11 g l−1 xylose and 5 g l−1 glucose initially gave the best result of 10.1 g l−1d-ribose concentration, 0.24 g g−1d-ribose yield and 0.29 g l−1 h−1 productivity, corresponding to 40-, 5- and 12-fold increases compared with those in the batch culture. A kinetic study of d-ribose production in fed-batch cultivations of B. subtilisJY200 suggested that xylose uptake might be critical to maximize d-ribosebiosynthesis from xylose.

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