site > International journal > (2005) Batch and fed-batch production of coenzyme Q10 in recombinant Escherichia coli containing decaprenyl diphosphate synthase gene from Gluconobacter suboxydans

Dept. of Advanced Fermentation Fusion Science & Technology, Kookmin University

|
회원가입

Topics

Professor | Current members | Alumni

Topics

International | Domestic | Patent

HOME > PUBLICATIONS

PUBLICATIONS

작성일 : 12-07-17 17:28

(2005) Batch and fed-batch production of coenzyme Q10 in recombinant Escherichia coli containing decaprenyl diphosphate synthase gene from Gluconobacter suboxydans

글쓴이 : 박용철

추천 : 0

http://www.springerlink.com/content/3me5a2d8ammet531/ [392]

Batch and fed-batch production of coenzyme Q10 in recombinant Escherichia coli containing decaprenyl diphosphate synthase gene from Gluconobacter suboxydans

Yong-Cheol Park, Soo-Jung Kim, Jin-Ho. Choi, Won-Heung Lee, Kyung-Moon. Park, Mokoto Kawamukai, Yeon-Woo Ryu and Jin-Ho Seo*
Applied Microbiology and Biotechnology 67(2): 192-196. 2005.04.01.
(SCI I/F:3.425)

Coenzyme Q10 (CoQ10) is a quinine consisting of ten units of the isoprenoid side-chain. Because it limits the oxidative attack of free radicals to DNA and lipids, CoQ10 has been used as an antioxidant for foods, cosmetics and pharmaceuticals. Decaprenyl diphosphate synthase (DPS) is the key enzyme for synthesis of the decaprenyl tail in CoQ10 with isopentenyl diphosphate. The ddsA gene coding for DPS from Gluconobacter suboxydans was expressed under the control of an Escherichia coli constitutive promoter. Analysis of the cell extract in recombinant E. coli BL21/pACDdsA by high performance liquid chromatography and mass spectrometry showed that CoQ10 rather than endogenous CoQ8 was biologically synthesized as the major coenzyme Q. Expression of the ddsA gene with low copy number led to the accumulation of CoQ10 to 0.97 mg l–1 in batch fermentation. A high cell density (103 g l–1) in fed-batch fermentation of E. coli BL21/pACDdsA increased the CoQ10 concentration to 25.5 mg l –1 and its productivity to 0.67 mg l–1 h–1, which were 26.0 and 6.9 times higher than the corresponding values for batch fermentation.

Contact Us

Science Building Room No. 204-205, Kookmin University
861-1 Jeongrung-dong, Sungbuk-gu, Seoul 136-702
Tel: +82-2-910-5596(5462), Fax: +82-2-910-5739
E-mail: ycpark@kookmin.ac.kr