site > International journal > (2003) Expression of Azotobacter vinelandii soluble transhydrogenase perturbs xylose reductase-mediated conversion of xylose to xylitol by recombinant Saccharomyces cerevisiae.

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작성일 : 12-07-17 14:15

(2003) Expression of Azotobacter vinelandii soluble transhydrogenase perturbs xylose reductase-mediated conversion of xylose to xylitol by recombinant Saccharomyces cerevisiae.

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http://www.sciencedirect.com/science/article/pii/S1381117703002297 [1097]

Expression of Azotobacter vinelandii soluble transhydrogenase perturbs xylose reductase-mediated conversion of xylose to xylitol by recombinant Saccharomyces cerevisiae.

Young-Suk Jeun, Myoung-Dong Kim, Yong-Cheol Park, Tae-Hee Lee, Myung-Sang Yoo, Yeon-Woo Ryu and Jin-Ho Seo*
Journal of Molecular Catalysis B: Enzymatic 26(3-6): 251-256. 2003.12.01.
(SCI I/F: 2.735)

Pyridine nucleotide transhydrogenase is a metabolic enzyme transferring the reducing equivalent between two nucleotide acceptors such as NAD+ and NADP+ for balancing the intracellular redox potential. Solubletranshydrogenase (STH) of Azotobactervinelandii was expressed in a recombinantSaccharomycescerevisiae strain harboring the Pichia stipitisxylosereductase (XR) gene to study effects of redox potential change on cell growth and sugar metabolism including xylitol and ethanol formation. Remarkable changes were not observed by expression of the STH gene in batch cultures. However, expression of STH accelerated the formation of ethanol in glucose-limited fed-batch cultures, but reduced xylitol productivity to 71% compared with its counterpart strain expressing xylosereductase gene alone. The experimental results suggested that A. vinelandii STH directed the reaction toward the formation of NADH and NADP+ from NAD+ and NADPH, which concomitantly reduced the availability of NADPH for xyloseconversion to xylitol catalyzed by NADPH-preferable xylosereductase in the recombinantS. cerevisiae.

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