site > International journal > (2001) Effects of environmental factors on in vivo folding of Bacillus macerans cyclodextrin glycosyltransferase in recombinant Escherichia coli.

Dept. of Advanced Fermentation Fusion Science & Technology, Kookmin University

|
회원가입

Topics

Professor | Current members | Alumni

Topics

International | Domestic | Patent

HOME > PUBLICATIONS

PUBLICATIONS

작성일 : 12-07-17 14:09

(2001) Effects of environmental factors on in vivo folding of Bacillus macerans cyclodextrin glycosyltransferase in recombinant Escherichia coli.

글쓴이 : 박용철

추천 : 0

http://cat.inist.fr/?aModele=afficheN&cpsidt=994493 [447]

Effects of environmental factors on in vivo folding of Bacillus macerans cyclodextrin glycosyltransferase in recombinant Escherichia coli.

Hee-Hyun Jin, Nam Soo Han, Dae-Hyuk Kweon, Yong-Cheol Park and Jin-Ho Seo*
Journal of Microbiology and Biotechnology 11(1): 92-96. 2001.02.28.
(SCI I/F: 1.381)

Effects of environmental factors on the expression of soluble forms of Bacillus macerans cyclodextrin glycosyltransferase in recombinant Escherichia coli BL21(DE3)pLysE:pTCGT1 were investigated. The amount of soluble CGTase produced in the cell was measured by determining its enzymatic activity. The soluble fraction of the enzyme was increased by lowering the culture temperature to 30°C and medium pH to 5.8 compared to the enzyme production in LB medium at 37C and pH 7.0. Addition of 0.2 M NaCl enhanced enzyme expression levels at the expense of cell growth. Glycine betaine that was added after 3 h of induction protected not only the cell growth from high osmotic pressure but also helped in vivo folding of CGTase in recombinant E. coli. Addition of 1 mM CaCl2 was also effective in the expression of soluble CGTase, resulting in 15 U/ml of the enzyme activity.

Contact Us

Science Building Room No. 204-205, Kookmin University
861-1 Jeongrung-dong, Sungbuk-gu, Seoul 136-702
Tel: +82-2-910-5596(5462), Fax: +82-2-910-5739
E-mail: ycpark@kookmin.ac.kr