site > International journal > (2011) Characterization of monoclonal antibody against aflatoxin B1 produced in hybridoma 2C12 and its single-chain variable fragment expressed in Escherichia coli.

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작성일 : 12-07-17 18:33

(2011) Characterization of monoclonal antibody against aflatoxin B1 produced in hybridoma 2C12 and its single-chain variable fragment expressed in Escherichia coli.

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http://www.sciencedirect.com/science/article/pii/S0308814610015062 [1156]

Characterization of monoclonal antibody against aflatoxin B1 produced in hybridoma 2C12 and its single-chain variable fragment expressed in Escherichia coli.

Won-Ki Min, Dae-Hyuk Kweon, Kyungmoon Park, Yong-Cheol Park* and Jin-Ho Seo
Food Chemistry. 126(3): 1316-1323. 2011.06.01
(SCI I/F: 3.655)

An anti-aflatoxin B1monoclonalantibody (anti-AFB1 mAb) from the hybridoma2C12 was established and its inhibition concentration fifty (IC50) for AFB1 and relative cross-reactivities (CRs) to other mycotoxins were estimated to be 8 ng/mL and less than 4% compared with AFB1 by a competitive direct enzyme-linked immunosorbent assay. For production of anti-AFB1single-chainvariablefragment (anti-AFB1 scFv) in recombinant Escherichiacoli, its scFv-coding genes were cloned from the hybridoma2C12. The anti-AFB1 scFv formed inclusion bodies in the cytoplasm of E. coli required in vitro refolding process and hence recovered to retain binding activity successfully. Surface plasmon resonance analysis resulted that anti-AFB1 scFv possessed 1.16 × 10−7 M of equilibrium dissociation constant (KD), which was about 17 times higher than the parental anti-AFB1 mAb of 6.95 × 10−9 M.

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