site > International journal > (2011) Expression of aldehyde dehydrogenase 6 reduces inhibitory effect of furan derivatives on cell growth and ethanol production in Saccharomyces cerevisiae

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작성일 : 12-07-17 18:31

(2011) Expression of aldehyde dehydrogenase 6 reduces inhibitory effect of furan derivatives on cell growth and ethanol production in Saccharomyces cerevisiae

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http://www.sciencedirect.com/science/article/pii/S0960852411003075 [1088]

Expression of aldehyde dehydrogenase 6 reduces inhibitory effect of furan derivatives on cell growth and ethanol production in Saccharomyces cerevisiae. Bioresource Technology.

Seong-Eon Park, Hyun Min Koo, Young Kyoung Park, Sung Min Park, Jae Chan Park, Oh-Kyu Lee, Yong-Cheol Park* and Jin-Ho Seo
Bioresource Technology. 102(10): 6033-6038. 2011.05.01
(SCI I/F: 4.980)

Yeast dehydrogenases and reductases were overexpressed in Saccharomycescerevisiae D452-2 to detoxify 2-furaldehyde (furfural) and 5-hydroxymethyl furaldehyde (HMF), two potent toxic chemicals present in acid-hydrolyzed cellulosic biomass, and hence improve cellgrowth and ethanolproduction. Among those enzymes, aldehydedehydrogenase6 (ALD6) played the dual roles of direct oxidation of furanderivatives and supply of NADPH cofactor to their reduction reactions. Batch fermentation of S. cerevisiae D452-2/pH-ALD6 in the presence of 2 g/L furfural and 0.5 g/L HMF resulted in 20–30% increases in specific growth rate, ethanol concentration and ethanol productivity, compared with those of the wild type strain. It was proposed that overexpression of ALD6 could recover the yeast cell metabolism and hence increase ethanolproduction from lignocellulosic biomass containing furan-derived inhibitors.

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