site > International journal > (2009) Synergistic effects of chromosomal ispB deletion and dxs overexpression on coenzyme Q10 production in recombinant Escherichia coli expressing Agrobacterium tumefaciens dps gene.

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작성일 : 12-07-17 18:09

(2009) Synergistic effects of chromosomal ispB deletion and dxs overexpression on coenzyme Q10 production in recombinant Escherichia coli expressing Agrobacterium tumefaciens dps gene.

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http://www.sciencedirect.com/science/article/pii/S0168165609001837 [1157]

Synergistic effects of chromosomal ispB deletion and dxs overexpression on coenzyme Q10 production in recombinant Escherichia coli expressing Agrobacterium tumefaciens dps gene.

Jin-Ho Choi, Yeon-Woo Ryu, Yong-Cheol Park* and Jin-Ho Seo*
Journal of Biotechnology 144(1): 64-69 2009.10.12.
(SCI I/F: 3.045)

For biotechnological production of coenzyme Q10 (CoQ10) in recombinantEscherichiacoli, three genetic manipulations were performed: heterologous expression of decaprenyl diphosphate synthase (Dps) from Agrobacteriumtumefaciens, deletion of endogenous octaprenyl diphosphate synthase (IspB), and overexpression of 1-deoxy-d-xylulose synthase (Dxs). Expression of the dpsgene and deletion of the ispBgene in E. coli BL21(DE3)ΔispB/pAP1 allowed production of CoQ10 only. Furthermore, coexpression of the dxsgene increased the specific content of CoQ10 from 0.55–0.89 mg g−1 to 1.40 mg g−1. For mass production of CoQ10, fed-batch fermentation of E. coli BL21(DE3)ΔispB/pAP1 + pDXS was carried out in a defined medium with 20 g l−1 initial glucose and by the glucose-feeding strategy of pH-stat. Finally, 99.4 mg l−1 CoQ10 concentration, 1.41 mg g−1 specific CoQ10 content and 3.11 mg l−1 h−1 productivity were obtained in 33 h of the fermentation, which were 78, 1.9, and 19 times higher than those for E. coli BL21(DE3)/pAP1 without the ispBdeletion and dxsoverexpression.

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