site > International journal > (2007) Effects of temperature shift strategies on production of human preproinsulin in fed-batch fermentation of recombinant Escherichia coli.

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작성일 : 12-07-17 17:42

(2007) Effects of temperature shift strategies on production of human preproinsulin in fed-batch fermentation of recombinant Escherichia coli.

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http://www.springerlink.com/content/y21x834015300ht5/ [399]

Effects of temperature shift strategies on production of human preproinsulin in fed-batch fermentation of recombinant Escherichia coli.

Young-Jin Son, Kyong-Hee Park, Sang-Yong Lee, Sung-Jin Oh, Chang-Kyu Kim, Byoung-Taek Choi, Yong-Cheol Park and Jin-Ho Seo*
Biotechnology and Bioprocess Engineering 12(5): 556-561. 2007.10.31.
(SCIE I/F: 1.278)

Preproinsulin is a well-known precursor of human insulin for the regulation of blood glucose levels. In this study, fed-batch fermentations of recombinantEscherichia coli JM109/pPT-MRpi were carried out for the overexpression of human preproinsulin. The expression of human preproinsulin was controlled by the temperature inducibleP2 promoter. The time-course profiles of fed-batch fermentation and SDS-PAGE analysis showed that human insulin expression was triggered by a culture temperature change from 30 to 37°C. Fermentation shift strategies, including the multi-step increase of temperature and the modulation of initiation time, were optimized to obtain high titers of cell mass and preproinsulin. The optimized fed-batch fermentation, consisting of a three-step shift of culture temperature from 30 to 37°C for 2 h, gave the best results of 43.1 g/L of dry cell weight and 33.3% preproinsulin content, which corresponded to 2.0- and 1.2-fold increases, respectively, as compared to those of fed-batch culture at a constant temperature of 37°C.

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