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작성일 : 12-09-11 08:43

(2013, IF=2.756) Production of 2,3-butanediol by a low-acid producing Klebsiella oxytoca NBRF4

글쓴이 : ycpark

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http://dx.doi.org/10.1016/j.nbt.2012.09.004 [1222]

Production of 2,3-butanediol by a low-acid producing Klebsiella oxytoca NBRF4

Sung-Hyuk Han1, Jung-Eun Lee, Kyungmoon Park and Yong-Cheol Park*

New Biotechnology 30(2): 166-172. 2013.01.25 (SCI IF=2.756)

Abstract
2,3-Butanediol (2,3-BDO) is a value-added chemical with great potential for the industrial production of synthetic rubber, plastic and solvent. For microbial production of 2,3-BDO, in this study, Klebsiella oxytoca NBRF4 was constructed by chemical mutation and screening against NaBr, NaBrO3 and fluoroacetate. Among metabolic enzymes involved in the production of lactate, acetate and 2,3-BDO, K. oxytoca NBRF4 possessed 1.2 times lower specific activities of lactate dehydrogenase and phosphotransacetylase, and 22% higher specific acetoin reductase activity than the K. oxytoca ATCC43863 control strain. A series of batch fermentations in a defined medium and application of a statistical tool of response surface method led to the determination of optimal culture conditions: 10% dissolved oxygen level, pH 4.3 and 38oC. The actual results of batch fermentation at the optimal conditions using 44 g/L glucose were coincident with the predetermined values: 14.4 g/L 2,3-BDO concentration, 0.32 g/g yield. To increase 2,3-BDO titer, fed-batch fermentation of K. oxytoca NBRF4 was performed by an intermittent feeding of 800 g/L glucose to control its concentration around 5-20 g/L in the culture broth. Finally, 34.2 g/L 2,3-BDO concentration and 0.35 g/g yield were obtained without organic acid production in 70 hrs of the fed-batch culture, which were 2.4 and 1.2 times higher than those of the batch fermentation using 44 g/L glucose.

Key words: 2,3-butanediol, Klebsiella oxytoca, response surface method, fed-batch fermentation

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