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(2012) Comparison of ethanol fermentation properties between laboratorial and industrial yeast strains using cassava hydrolysate

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http://www.kjmb.or.kr/journal/view2.html?book=Journal&tops=&start=0&sc… [523]

Comparison of ethanol fermentation properties between laboratorial and industrial yeast strains using cassava hydrolysate

Young-Wook Chin, Jin-Woo Kim, Yong-Cheol Park* and Jin-Ho Seo
Korean Journal of Microbiology and Biotechnology 40(3): 220~225, (2012.9.30) (SCOPUS)

Abstract

To investigate the ethanol fermentation properties of alcohol yeasts, in this study, a laboratorial strain (CEN.PK2-1D) and two industrial alcohol yeasts (JHS100 and JHS200) of Saccharomyces cerevisiae were cultured in a pure YP medium with 300 g/L glucose and cassava hydrolysate only. Spot assay and cell viability test showed that both JHS100 and JHS200 strains showed higher ethanol tolerance than the CEN.PK2-1D strain. Among them, the JHS100 strain gained the highest performance of cell growth, glucose consumption and ethanol production. Especially, an anaerobic batch fermentation of the JHS100 strain using cassava hydrolysate only with 250 g/L glucose resulted in 106.1 g/L ethanol concentration, 0.42 g/g ethanol yield and 3.15 g/L-hr ethanol productivity, which were 53%, 13%, 53% higher than the corresponding values for the CEN.PK2-1D strain. By changing pure YP medium to cassava hydrolysate, 19% and 17% decreases in ethanol yield and productivity for the CEN.PK2-1D strain were observed, whereas the cultures of the JHS100 and JHS200 stains showed similar ethanol productivities and only an 8% decrease in ethanol yield. Furthermore, the JHS100 and JHS200 stains produced lower levels of glycerol and acetate byproducts than the CEN.PK2-1D strain. Consequently, the outstanding ethanol fermentation performance of the industrial strains might be owing to rapid cell growth, high ethanol tolerance, low nitrogen requirement and low formation of by-products.

Key words: ethanol, Saccharomyces cerevisiae, laboratorial yeast, industrial yeast, cassava hydrolysate

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