site > International journal > (2012, IF=3.045) Effects of NADH-preferring xylose reductase expression on ethanol production from xylose in xylose-metabolizing recombinant Saccharomyces cerevisiae.

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작성일 : 12-07-18 08:59

(2012, IF=3.045) Effects of NADH-preferring xylose reductase expression on ethanol production from xylose in xylose-metabolizing recombinant Saccharomyces cerevisiae.

글쓴이 : ycpark

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http://www.sciencedirect.com/science/article/pii/S016816561100304X [1115]

Effects of NADH-preferring xylose reductase expression on ethanol production from xylose in xylose-metabolizing recombinant Saccharomyces cerevisiae.

Sung-Haeng Lee, Tsutomu Kodaki, Yong-Cheol Park* and Jin-Ho Seo
Journal of Biotechnology. 158(4): 184-191, 2012.04.30
(SCI I/F: 3.045)

Abstract

Efficient conversion of xylose to ethanol is an essential factor for commercialization of lignocellulosic ethanol. To minimize production of xylitol, a major by-product in xylose metabolism and concomitantly improve ethanolproduction, Saccharomycescerevisiae D452-2 was engineered to overexpress NADH-preferable xylosereductase mutant (XRMUT) and NAD+-dependent xylitol dehydrogenase (XDH) from Pichia stipitis and endogenous xylulokinase (XK). In vitro enzyme assay confirmed the functional expression of XRMUT, XDH and XK in recombinantS. cerevisiae strains. The change of wild type XR to XRMUT along with XK overexpression led to reduction of xylitol accumulation in microaerobic culture. More modulation of the xylose metabolism including overexpression of XRMUT and transaldolase, and disruption of the chromosomal ALD6 gene encoding aldehyde dehydrogenase (SX6MUT) improved the performance of ethanolproduction from xylose remarkably. Finally, oxygen-limited fermentation of S. cerevisiae SX6MUT resulted in 0.64 g l−1 h−1xylose consumption rate, 0.25 g l−1 h−1ethanol productivity and 39% ethanol yield based on the xylose consumed, which were 1.8, 4.2 and 2.2 times higher than the corresponding values of recombinantS. cerevisiae expressing XRMUT, XDH and XK only.

Key words: Ethanol; Xylose; Saccharomyces cerevisiae; NADH-preferable xylose reductase

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