site > International journal > (2012) Effect of overexpression of acetaldehyde dehydrogenase 6 and acetyl-CoA synthetase 1 on xylitol production in recombinant Saccharomyces cerevisiae.

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작성일 : 12-07-18 08:55

(2012) Effect of overexpression of acetaldehyde dehydrogenase 6 and acetyl-CoA synthetase 1 on xylitol production in recombinant Saccharomyces cerevisiae.

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http://www.sciencedirect.com/science/article/pii/S1878818111000120 [993]

Effect of overexpression of acetaldehyde dehydrogenase 6 and acetyl-CoA synthetase 1 on xylitol production in recombinant Saccharomyces cerevisiae.

Eun-Jung Oh, Yi-hyun Bae, Kyoung Heon Kim, Yong-Cheol Park* and Jin-Ho Seo
Biocatalysis and Agricultural Biotechnology. 1:15-19.2012.1.1.

Supply of NAD(P)H is a key factor in microbial production of xylitol from xylose. To facilitate NAD(P)H supplementation, acetaldehydedehydrogenase6 (ALD6) and acetyl-CoAsynthetase1 (ACS1) were overexpressed in recombinantSaccharomycescerevisiae harboring the Pichia stipitis xylose reductase (XR) gene. In-vitro activity analysis confirmed the functional expression of both enzymes. Glucose-limited fed-batch fermentations of Saccharomycescerevisiae BJ3505:δXR strains overexpressing ACS1 and/or ALD6 were performed by feeding 600 g/L glucose in the presence of 100 g/L xylose. Among them, ACS1overexpression gave the best result of xylitolproduction: 91.3 g/L xylitol concentration and 1.76 g/L h xylitol productivity, which were 25% and 11% increase, relative to those of the control and ALD6-overexpressing strains. Considering the changes of cell growth, ethanol and acetate production, a remarkable enhancement of xylitolproduction by ACS1overexpression seemed be ascribed to energy and NAD(P)H generation through a metabolism from acetaldehyde to acetyl-CoA and TCA cycle.

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