site > International journal > (2009) Modulation of guanosine 5’-phosphate-D-mannose metabolism in recombinant Escherichia coli for production of guanosine 5’-phosphate-L-fucose.

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작성일 : 12-07-17 18:12

(2009) Modulation of guanosine 5’-phosphate-D-mannose metabolism in recombinant Escherichia coli for production of guanosine 5’-phosphate-L-fucose.

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http://www.sciencedirect.com/science/article/pii/S0960852409009055 [1158]

Modulation of guanosine 5’-phosphate-D-mannose metabolism in recombinant Escherichia coli for production of guanosine 5’-phosphate-L-fucose

Won-Heong Lee, Nam Soo Han Yong-Cheol Park* and Jin-Ho Seo*
Bioresource Technology 100(24): 6143-6148 2009.12.01.
(SCI I/F: 4.980)

Guanosine 5’-diphosphate (GDP)-l-fucose, an activated form of a nucleotide sugar, plays an important role in a wide range of biological functions. In this study, the enhancement of GDP-l-fucose production was attempted by supplementation of mannose, which is a potentially better carbon source to be converted into GDP-l-fucose than glucose, and combinatorial overexpression of the genes involved in the biosynthesis of GDP-d-mannose, a precursor of GDP-l-fucose. Supply of a mannose and glucose led to a 1.3-fold-increase in GDP-l-fucose concentration (52.5 ± 0.8 mg l−1) in a fed-batch fermentation of recombinant E. coli BL21star(DE3) overexpressing the gmd and wcaG genes, compared with the case using glucose as a sole carbon source. A maximum GDP-l-fucose concentration of 170.3 ± 2.3 mg l−1, corresponding to a 4.4-fold enhancement compared with the control strain overexpressing gmd and wcaG genes only, was achieved in a glucose-limited fed-batch fermentation of a recombinant E. coli BL21star(DE3) strain overexpressing manB, manC, gmd and wcaG genes. Further improvement of GDP-l-fucose production was not obtained by additional overexpression of the manA gene.

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