site > International journal > (2015, IF=2.871) Production of (S)-3-hydroxybutyrate by metabolically engineered Saccharomyces cerevisiae

Dept. of Advanced Fermentation Fusion Science & Technology, Kookmin University

|
회원가입

Topics

Professor | Current members | Alumni

Topics

International | Domestic | Patent

HOME > PUBLICATIONS

PUBLICATIONS

작성일 : 15-06-26 10:54

(2015, IF=2.871) Production of (S)-3-hydroxybutyrate by metabolically engineered Saccharomyces cerevisiae

글쓴이 : ycpark

추천 : 0

http://www.sciencedirect.com/science/article/pii/S016816561530002X [1128]

Production of (S)-3-hydroxybutyrate by metabolically engineered Saccharomyces cerevisiae

Eun Ju Yun, Suryang Kwak, Soo Rin Kim, Yong-Cheol Park, Yong-Su Jin,* Kyoung Heon Kim*

Journal of Biotechnology 209:23-30 (2015. 9.10) (IF=2.871)

(S)-3-Hydroxybutyrate (S-3HB) can be used as a precursor for the synthesis of biodegradable polymerssuch as polyhydroxyalkanoate and stereo-specific fine chemicals such as antibiotics, pheromones, anddrugs. For the production of S-3HB in yeast, the biosynthetic pathway of S-3HB from acetyl-CoA, consistingof the three enzymes, acetyl-CoA C-acetyltransferase (ACCT), acetoacetyl-CoA reductase (ACR), and 3-hydroxybutyryl-CoA thioesterase (HBT), was introduced into Saccharomyces cerevisiae. An engineeredyeast strain overexpressing ERG10, hbd, and tesB genes not only exhibited enzyme activities of AACT,ACR, and HBT, but also produced S-3HB from ethanol. In order to increase the titer of S-3HB, a fed-batchfermentation based on pulse feeding of ethanol as a carbon source was performed, and a final S-3HB titerof 12.0 g/L was achieved. This is the first report on the production of 3HB by engineered yeast, utilizingethanol as the carbon source, suggesting that the industrially preferred S. cerevisiae can be a promisinghost for producing S-3HB.

Contact Us

Science Building Room No. 204-205, Kookmin University
861-1 Jeongrung-dong, Sungbuk-gu, Seoul 136-702
Tel: +82-2-910-5596(5462), Fax: +82-2-910-5739
E-mail: ycpark@kookmin.ac.kr