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작성일 : 15-03-12 22:49

(2015, IF=4.494) Evolutionary engineering of Saccharomyces cerevisiae for efficient conversion of red algal biosugars to bioethanol

글쓴이 : ycpark

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http://dx.doi.org/10.1016/j.biortech.2015.03.057 [1187]

Evolutionary engineering of Saccharomyces cerevisiae for efficient conversion of red algal biosugars to bioethanol

Hye-Jin Lee, Soo-Jung Kim, Jeong-Jun Yoon, Kyoung Heon Kim, Jin-Ho Seo and Yong-Cheol Park*

Bioresource Technology, 191:445-451 (2015.9)

Abstract
The aim of this work was to apply the evolutionary engineering to construct a mutant Saccharomyces cerevisiae HJ7-14 resistant on 2-deoxy-D-glucose and with an enhanced ability of bioethanol production from galactose, a mono-sugar in red algae. In batch and repeated-batch fermentations, HJ7-14 metabolized 5-fold more galactose and produced ethanol 2.1-fold faster than the parental D452-2 strain. Transcriptional analysis of genes involved in the galactose metabolism revealed that moderate relief from the glucose-mediated repression of the transcription of the GAL genes might enable HJ7-14 to metabolize galactose rapidly. HJ7-14 produced 7.4 g/L ethanol from hydrolysates of the red alga Gelidium amansii within 12 h, which was 1.5-times faster than that observed with D452-2. We demonstrate conclusively that evolutionary engineering is a promising tool to manipulate the complex galactose metabolism in S. cerevisiae to produce bioethanol from red alga.

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