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작성일 : 14-10-16 10:38

(2014, IF=3.708) One-pot pretreatment, saccharification, and ethanol fermentation of lignocelluose based on acid-base mixture pretreatment

글쓴이 : ycpark

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http://pubs.rsc.org/en/content/articlelanding/2014/ra/c4ra10092a/unaut… [474]

One-pot pretreatment, saccharification, and ethanol fermentation of lignocelluose based on acid-base mixture pretreatment

Young Hoon Jung, Hyun Min Park, In Jung Kim, Yong-Cheol Park, Jin-Ho Seo and Kyoung Heon Kim

RSC Advances 2014, 4, 55318-55327

Abstract
Currently, for the production of cellulosic ethanol, multi-step unit operations, including pretreatment, solid/liquid (S/L) separation, solids washing, liquid detoxification, neutralization, enzymatic hydrolysis and fermentation, are the commonly required steps, which contribute to the elevated capital and operating costs. To simplify these steps, consolidated bioprocessing (CBP), focusing on the multi-functional microbial strains, was proposed but still far from industrialization. In this study, using an acid-base mixture as a pretreatment catalyst, pretreatment, saccharification and fermentation were performed in one pot without S/L separation, neutralization and detoxification. From the one-pot process based on the acid-base mixture pretreatment (190°C, 2 min and 0.15 (w/v) acid‒base mixture) and 15 FPU of cellulase/g glucan and Sacchromyces cerevisiae, 70.7% of the theoretical maximum ethanol yield (based on the initial amount of glucan in untreated rice straw) was obtained. This was comparable to the estimated ethanol yield (e.g. 72.9%) from the assumption of 90% glucan recovery yield after pretreatment × 90% glucose yield from saccharification × 90% ethanol yield from ethanol fermentation, which are performed in three separate pots. These results suggest that whole slurry processing of lignocellulose in one pot could be an attractive way to achieve economic sustainability in the production of fuel from lignocellulose.

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