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작성일 : 14-01-03 22:57

(2014, IF=4.980) Enhanced production of 3-hydroxypropionic acid from glycerol by modulation of glycerol metabolism in recombinant Escherichia coli

글쓴이 : ycpark

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http://dx.doi.org/10.1016/j.biortech.2014.01.009 [1174]

Enhanced production of 3-hydroxypropionic acid from glycerol by modulation of glycerol metabolism in recombinant Escherichia coli

Kwangwook Kim1, Sun-Ki Kim2, Yong-Cheol Park3,** and Jin-Ho Seo1,2,*

Bioresource Technology, 156: 170-175, 2014.03. (SCI IF=4.980, Journal ranking category (Energy&Fuel)= 7/81)

Abstract
3-Hydroxypropionic acid (3-HP) is a valuable biochemical with high potential for bioplastic manufacturing. The endogenous glycerol metabolism and by-product formation pathway in Escherichia coli were modulated to enhance 3-HP production from glycerol. Double deletion of glpK and yqhD directed the glycerol flux to 3-HP biosynthesis and reduced the formation of 1,3-propanediol. Since 3-hydroxypropionaldehyde (3-HPA), a precursor of 3-HP, is toxic to cell growth, the gene encoding Pseudomonas aeruginosa semialdehyde dehydrogenase (PSALDH) highly active on 3-HPA was expressed in E. coli. Finally, fed-batch culture of recombinant E. coli BL21star(DE3) without glpK and yqhD, and expressing Lactobacillus brevis DhaB-DhaR, and P. aeruginosa PSALDH resulted in 57.3 g/L 3-HP concentration, 1.59 g/L-h productivity and 0.88 g/g yield. In conclusion, modulation of the glycerol metabolism in combination with enhanced activity of 3-HPA dehydrogenation improved the production of 3-HP from glycerol.

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