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작성일 : 12-11-12 08:59

(2013, IF=4.980) Biosynthesis of 3-hydroxypropionic acid from glycerol in recombinant Escherichia coli expressing Lactobacillus brevis dhaB and dhaR gene clusters and E. coli K-12 aldH

글쓴이 : ycpark

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http://dx.doi.org/10.1016/j.biortech.2012.11.063 [1247]

Biosynthesis of 3-hydroxypropionic acid from glycerol in recombinant Escherichia coli expressing Lactobacillus brevis dhaB and dhaR gene clusters and E. coli K-12 aldH

Suryang Kwak, Yong-Cheol Park* and Jin-Ho Seo

Bioresource Technology (SCI IF=4.980, Journal ranking category (Energy&Fuel)= 7/81)
vol. 135 pages 432-439, 2013.05.01

Abstract

3-Hydroxypropionic acid (3-HP) is a value-added chemical used for polymer synthesis. For microbial production of 3-HP from glycerol, two dhaB and dhaR gene clusters encoding glycerol dehydratase (DhaB) and its reactivating factor (DhaR), respectively, were cloned from Lactobacillus brevis KCTC33069 and expressed in Escherichia coli. Mass spectrometric analysis revealed that DhaB possessed glycerol dehydratase activity. DhaR expression increased DhaB activity 1.4-fold and allowed the recombinant E. coli to convert glycerol to 3-hydroxypropionaldehyde, an intermediate of 3-HP biosynthesis. To produce 3-HP from glycerol, a fed-batch fermentation of recombinant E. coli JHS00947 producing L .brevis DhaB and DhaR, and E. coli K-12 AldH was carried out by a two-step feeding strategy, separating the cell growth from the 3-HP production stages. A 3-HP concentration of 14.3 g/L and a productivity of 0.26 g/L-h were obtained in the fed-batch fermentation. These values were 14.6 and 8.53 times higher than the corresponding values of the batch culture. It was concluded that the cloning and overexpression of L. brevis dhaB and dhaR clusters and E. coli aldH, in combination with implementation of the two-step feeding strategy enabled recombinant E. coli to convert glycerol to 3-HP efficiently.

Key words: 3-hydroxypropionic acid, Escherichia coli, glycerol dehydratase, glycerol dehydratase reactivating factor, Lactobacillus brevis

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