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작성일 : 15-02-20 17:37

(2015, IF=1.525) Fast determination of multiple-reaction intermediates for long-chain dicarboxylic acid production by gas chromatography-flame ionization detector

글쓴이 : ycpark

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http://www.jmb.or.kr/journal/viewJournal.html?doi=10.4014/jmb.1502.020… [465]

Fast determination of multiple-reaction intermediates for long-chain dicarboxylic acid production
by gas chromatography-flame ionization detector

Yong-Han Cho, Hye-Jin Lee, Jeung-Eun Lee, Soo-Jung Kim, Kyungmoon Park, Do Yup Lee and
Yong-Cheol Park*

Journal of Microbiology and Biotechnology, 25(5):704-708 (2015.5.28)

For the analysis of multiple-reaction intermediates for long-chain dicarboxylic acid biotransformation, simple and reproducible methods of extraction and derivatization were developed on the basis of gas chromatography with flame ionization detector (GC-FID) instead of mass spectrometer (MS). In the derivatization step, change of the ratio of pyridine to MSTFA from 1:3 to 9:1 resulted in higher peak intensity (p = 0.021) and reproducibility (0.6%CV) when analyzing 32 g/L ricinoleic acid (RA). Extraction of RA and ω-hydroxyundec-9-enoic acid (ω-HUA) with water containing 100 mM tween80 showed 90.4~99.9% relative extraction efficiency and 2~7%CV compared to those with hydrophobic ethyl acetate. In conclusion, reduction of pyridine content and change of extraction solvent to water with tween80 provided compatible derivatization and extraction methods to GC-FID-based analysis of long-chain carboxylic acids.

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