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작성일 : 17-02-03 10:40

(2017, IF=2.529) Production of D-ribose by metabolically engineered Escherichia coli

글쓴이 : ycpark

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http://dx.doi.org/10.1016/j.procbio.2016.10.001 [1113]

Production of D-ribose by metabolically engineered Escherichia coli

Hae-Chul Park, Yun-Jung Kim, Chang-Wan Lee, Yong-Taek Rho, JeongWoo Kang, Dae-Hee Lee, Yeong-Je Seong, Yong-Cheol Park, Daesang Lee, Sung-Gun Kim

Process Biochemistry 52:73-77 (2017.01)

Abstract
Escherichia coli was metabolically engineered for the production of d-ribose, a functional five-carbon sugar, from xylose. For the accumulation of d-ribose, two genes of transketolase catalyzing the conversion of d-ribose-5-phosphate to sedoheptulose-7-phosphate in pentose phosphate pathway were disrupted to create a transketolase-deficient E. coli SGK013. In batch fermentation, E. coli SGK013 grew by utilizing glucose and then started to produce d-ribose from xylose after glucose depletion. E. coli SGK013 produced 0.75 g/L of d-ribose, which was identical to the standard d-ribose as confirmed by HPLC and LC/MS analyses. To improve D-ribose production, the ptsG gene encoding the glucose-specific IICB component was disrupted additionally, resulting in the construction of E. coli SGK015. The carbon catabolite repression-negative E. coli SGK015 utilized xylose and glucose simultaneously and produced up to 3.75 g/L of d-ribose, which is a 5-fold improvement compared to that of E. coli SGK013.

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