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작성일 : 21-01-03 12:27

(2020, IF=3.998) Development of fluorescent Escherichia coli for a whole-cell sensor of 2'-fucosyllactose

글쓴이 : ycpark

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https://www.nature.com/articles/s41598-020-67359-x [414]

Development of fluorescent Escherichia coli for a whole-cell sensor of 2'-fucosyllactose

Jonghyeok Shin, Myungseo Park, Chakhee Kim, Hooyeon Kim, Yunjeong Park, Choongjin Ban, Jong-Won Yoon, Chul-Soo Shin, Jae Won Lee, Yong-Su Jin, Yong-Cheol Park, Won-Ki Min* and Dae-Hyuk Kweon

Scientific Reports 10: 10514 (2020.06.29)

2′-Fucosyllactose (2′-FL), a major component of fucosylated human milk oligosaccharides, is beneficial to human health in various ways like prebiotic effect, protection from pathogens, anti-inflammatory activity and reduction of the risk of neurodegeneration. Here, a whole-cell fluorescence biosensor for 2′-FL was developed. Escherichia coli (E. coli) was engineered to catalyse the cleavage of 2′-FL into L-fucose and lactose by constitutively expressing α-L-fucosidase. Escherichia coli ∆L YA, in which lacZ is deleted and lacY is retained, was employed to disable lactose consumption. E. coli ∆L YA constitutively co-expressing α-L-fucosidase and a red fluorescence protein (RFP) exhibited increased fluorescence intensity in media containing 2′-FL. However, the presence of 50 g/L lactose reduced the RFP intensity due to lactose-induced cytotoxicity. Preadaptation of bacterial strains to fucose alleviated growth hindrance by lactose and partially recovered the fluorescence intensity. The fluorescence intensity of the cell was linearly proportional to 1–5 g/L 2′-FL. The whole-cell sensor will be versatile in developing a 2′-FL detection system.

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