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작성일 : 25-05-29 11:47

(2025, IF=9.7) Enzyme cascades for high-yield conversion of D-xylose into D-ribose by overcoming equilibrium constraints and enhancing selectivity

글쓴이 : ycpark

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https://www.sciencedirect.com/science/article/pii/S0960852425004018 [0]

Enzyme cascades for high-yield conversion of D-xylose into D-ribose by overcoming equilibrium constraints and enhancing selectivity

Ja Hyun Lee, Doyeon Kim, Yoonjoo Kim, Dong Hyun Kim*, Yong-Cheol Park* and Kyoung Heon Kim*

Bioresource Technology, 428:132435 (2025.07.01)

d-Ribose is essential for critical cellular functions and the synthesis of antiviral nucleosides. However, traditional chemical synthesis and fermentation methods of d-ribose production suffer from low yields and inefficient resource utilization. Here, we present a highly efficient enzymatic cascade strategy that utilizes selective phosphorylation and dephosphorylation processes, coupled with ATP regeneration to convert d-xylose into d-ribose with high yield. By optimizing this enzyme cascade, we achieved a substantial increase in d-ribose yield from 23.4 % to 93.5 % mol/mol, effectively overcoming the equilibrium limitations of sugar conversion processes. Notably, our approach allows for the selective conversion of d-xylose to d-ribose in lignocellulosic hydrolysates, even in the presence of d-glucose. This work demonstrates the highly efficient enzymatic conversion of d-xylose into d-ribose offering a competitive alternative to existing chemical synthesis methods. Our findings provide a novel approach to cellulosic biomass valorization and represent a significant contribution to the field of biorefinery.

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